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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Althea Medical Journal Medical Journal of Indonesia Global Medical and Health Communication The Indonesian Biomedical Journal Sari Pediatri Journal of Medicine and Health Majalah Kedokteran Andalas Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Neuroanestesi Indonesia

Ida Parwati

Department Of Clinical Pathology, Faculty Of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital, Bandung

Author-ID : 340676

Biochemistry, Genetics & Molecular Biology Dentistry Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Other

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High ESAT-6 Expression in Granuloma Necrosis Type of Tuberculous Lymphadenitis Wida Purbaningsih; Djatnika Setiabudi; Herri S. Sastramihardja; Ida Parwati
Global Medical & Health Communication (GMHC) Vol 6, No 2 (2018)
Publisher : Universitas Islam Bandung

A granuloma is one of host cellular immune response form to intracellular and persistent pathogens, and result in the aggregation of several activated immune cells. Intracellular pathogens manipulate host immune responses to avoid immune reactions. M. tuberculosis is the intracellular and persister pathogen, which can stimulate granuloma formation. The formation this granulomas still have different opinions, whether it is the host's way to isolate M. tuberculosis, or how these pathogens are to escape immune responses. Early secretory antigenic target (ESAT)-6 is a typical secretory protein produced by the locus of the gene region of difference (RD)-1 M. tuberculosis. ESAT-6 plays a role in the immunopathogenesis of tuberculosis. This study aims to compare ESAT-6 antigen expression from M. tuberculosis between granulomas with necrosis and granulomas without necrosis. This study was an analytic observation study with a cross-sectional design. Forty-six lymph node paraffin blocks from tuberculous lymphadenitis patients in Department of Anatomical Pathology, Dr. Hasan Sadikin General Hospital, Bandung in 2017 were made in preparations and stained by hematoxylin eosin to assess the presence of necrosis in granulomas, immunohistochemical using ESAT-6 antibodies, then it was quantified using histoscore. Histoscore for ESAT-6 not normally distributed, so it uses Mann-Whitney test used. The results showed that there were 31 granulomas with necrosis (histoscore mean=27.6%) and 15 granulomas without necrosis (histoscore mean=15.1%), there was a significant difference with p<0.05 (p=0.03). The conclusion of this study there is a high histoscore ESAT-6 expression in granuloma type of necrosis tuberculous lymphadenitis. EKPRESI ESAT-6 TINGGI PADA GRANULOMA LIMFADENITIS TUBERKULOSIS TIPE NEKROSISGranuloma merupakan salah satu bentuk respons imun seluler pejamu terhadap patogen intraseluler. Patogen intraseluler memanipulasi respons imun pejamu untuk menghindari reaksi imun. M. tuberculosis adalah patogen intraseluler dan persister yang dapat menstimulasi pembentukan granuloma. Terbentuknya granuloma masih memberikan pendapat yang berbeda, apakah merupakan cara tubuh untuk mengisolasi M. tuberculosis atau cara patogen ini untuk menghindari respons imun. Early secretory antigenic target (ESAT)-6 adalah protein sekretori khas yang dihasilkan oleh lokus gen region of difference (RD)-1 M. tuberculosis. ESAT-6 berperan dalam imunopatogenesis tuberkulosis. Penelitian ini bertujuan menganalisis perbedaan ekspresi antigen ESAT-6 M. tuberculosis antara granuloma dengan nekrosis dan granuloma tanpa nekrosis. Penelitian ini merupakan penelitian observasi analitik dengan desain cross sectional. Blok parafin kelenjar getah bening didapat dari pasien yang didiagnosis limfadenitis tuberkulosis di Departemen Patologi Anatomi, RSUP Dr. Hasan Sadikin Bandung pada tahun 2017. Blok parafin tersebut dibuat blangko preparat dan diwarnai dengan hematoksilin eosin untuk menilai nekrosis pada granuloma serta imunohistokimia menggunakan antibodi ESAT-6. Kemudian, sediaan preparat imunohistokimia tersebut dikuantifikasi menggunakan metode histoscore sehingga didapatkan data berupa nilai skor dari pewarnaan ESAT-6. Selanjutnya, dilakukan uji beda antara histoscore granuloma dengan nekrosis dan granuloma tanpa nekrosis tersebut dianalisis karena nilai skor ESAT-6 berdistribusi tidak normal sehingga menggunakan uji Mann-Whitney. Hasil penelitian menunjukkan terdapat 31 granuloma dengan nekrosis (histoscore rerata=27,6%) dan 15 granuloma tanpa nekrosis (histoscore rerata=15,1%), serta terdapat perbedaan signifikan dengan p<0,05 (p=0,03). Simpulan, ekspresi ESAT-6 tinggi pada granuloma limfadenitis tuberkulosis dengan nekrosis.

Imunoekspresi ER-α, PR, Wnt5a, dan HMGA2 pada Berbagai Gradasi Tumor Filodes Payudara Fairuz Quzwain; Yusuf Sulaiman Effendi; Bethy Suryawati Hernowo; Ida Parwati
Global Medical & Health Communication (GMHC) Vol 4, No 2 (2016)
Publisher : Universitas Islam Bandung

Tumor filodes merupakan tumor fibroepitelial pada payudara yang secara mikroskopis ditandai dengan peningkatan selularitas sel stroma dan membentuk struktur seperti daun (leaf-like). Berbeda dengan tumor dari unsur epitel duktuli dan kelenjar payudara, penelitian peranan hormonal maupun faktor lain pada tumor filodes masih menunjukkan hasil yang inkonsisten sehingga menyebabkan patogenesis dan penatalaksanaan tumor ini dalam jalur hormonal masih kontroversi. Penelitian ini bertujuan menganalisis imunoekspresi faktor hormonal, yaitu estrogen receptor alpha (ER-α), progesteron receptor (PR), serta faktor nonhormonal HMGA2 dan wnt5a pada berbagai gradasi tumor filodes payudara. Dilakukan penilaian histologi dan imunoekspresi pada parafin blok jaringan tumor filodes payudara di Laboratorium Departemen Patologi Anatomi RSUP Dr. Hasan Sadikin Bandung periode tahun 2011 sampai 2014. Subjek penelitian dibagi menjadi 3 kelompok gradasi berdasarkan kriteria WHO tahun 2012, yaitu benign, borderline, dan malignant. Didapatkan 62 kasus tumor filodes yang sebagian besar menunjukkan distribusi imunoekspresi ER-α >50%, yaitu pada kategori benign sebanyak 35 dari 40 pasien. Terdapat korelasi signifikan histoskor ER-α, HMGA2, dan Wnt5a dengan gradasi histopatologis PT (p=0,002; p=0,001; p=0,040) dengan arah negatif untuk ER-α 2 (R=−0,423) serta positif untuk HMGA2 dan Wnt5a (R=0,439 dan R=0,243). Pada penelitian ini dapat disimpulkan semakin besar nilai histoskor ER-α maka semakin banyak diekpresikan pada gradasi benign dan semakin besar nilai histoskor HMGA2 serta Wnt5a semakin banyak ditemukan pada gradasi malignant.ER-α, PR, HMGA2 AND WNT5A IMMUNOEXPRESSION IN VARIOUS GRADE OF PHYLLODES TUMOR OF THE BREASTPhyllodes tumors (PTs) of the breast are fibroepithelial neoplasms, histologically characterized by hypercellular stromal, stromal overgrowth and double-layered epithelial component arranged in clefts which in combination elaborate leaf-like structures. Unlike epithelial neoplasm of the duct and gland of the breast, there were inconsitence and controversion in hormonal expression research in PTs. These were make unclear pathogenesis and unestablished hormonal therapy in PTs. The aim of this study was to analysis the immunoexpression estrogen receptor-alpha (ER-α), progesterone receptor (PR), HMGA and Wnt5a in various grade of phyllodes tumors of the breast. We reviewed histology and performed immunohistochemistry for paraffin block of PTs in Laboratory of Departement Pathological Anatomic at Dr. Hasan Sadikin General Hospital in 2011 until 2014 period. According to WHO classification (2012), PTs were categorized into three groups benign, borderline and malignant. According to 62 cases of PT, mainly showed that distribution of immunoexpresion ER-α >50% in benign category were 35 of 40 patients. A significant correlation was observed between histoscore ER-α, HMGA2 and Wnt5a (p=0.002, p=0.001, p=0.040) and histopathological grading of PT (p=0.001) in negative direction (R=−0.423), HMGA2 and Wnt5a in positive direction (R=0.439 and R=0.243). It indicates that the histoscore ER-a value increases with increasing expressed value in benign grade. On the other side, the histoscore HMGA2 and Wnt5a value increases with increasing expressed value in malignant grade.

Protective Effect of the T1212C Macrophage Mannose Receptor Gene Polymorphism on Pulmonary Tuberculosis Yani Triyani; Julia Hartati; Budiman Budiman; Ida Parwati; Bachti Alisjahbana
Global Medical & Health Communication (GMHC) Vol 10, No 3 (2022)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v10i3.10706

The interaction between the mannose receptor, which is encoded by the macrophage mannose receptor (MMR) gene, and the most virulent antigen (the mannose-capped lipoarabinomannan) cell wall of virulent strains of Mycobacterium tuberculosis trigger an innate and adaptive immune response. It also produces pro and anti-inflammatory cytokines, which play a role in the pathogenesis of tuberculosis (TB) infection. Therefore, MMR gene polymorphism is a risk factor associated with the prognosis for active pulmonary TB. This study aimed to determine the correlation between MMR gene polymorphism and active or latent pulmonary tuberculosis. In this phase, MMR gene polymorphism was analyzed using a case-control design consisting of 74 control group subjects (patients with latent TB) and 74 case groups (patients with active pulmonary TB). The subject’s MMR gene DNA sequencing examination. The study was conducted at the Teaching Hospital, Faculty of Medicine, Universitas Padjadjaran Bandung, from February 2014 to January 2015. The statistical analysis used chi-square and odds ratio. The study’s result has shown the MMR gene polymorphism factor that correlated to the incidence of active pulmonary TB was T1212C (OR=0.253; 95% CI=0.111−0.575; p=0.001). There was an MMR gene in one SNP in the control group (C1323T) only and five single nucleotide polymorphisms (SNPs) in both groups (C1303T, C1221, T1212C, G1186A, and G1195A). Therefore, it can be concluded that MMR gene polymorphism on the T1212C site correlated with the incidence of active pulmonary tuberculosis and was protective.

Co-Authors - Hidayat, - - Liza A K Sugianli A Rizal Abdul Hadi Martakusumah Adhi K Sugianli Adhi Kristianto Sugianli Adrian Suhendra Agnes Rengga I Agnes Rengga Indrati Ahmad Faried Ahmad Rizal Ahmad Rizal Ganiem Al Rasyid Amanda Widayanti Andani Eka Putra Andi Basuki Prima Birawa Andi Basuki Prima Birawa Andre van der Venn Anggra Filani Anggraini Alam Ani Melani Maskoen Anis Karuniawati Anna Tjandrawati Arto Yuwono Arto Yuwono Soeroto, Arto Yuwono Astrid Tamara Maajid Budiman Bachti Alisjabana, Bachti Bachti Alisjahbana Bachti Alisyahbana Basti Andriyoko Basti Andriyoko Bethy S. Hernowo Bethy Suryawati Hernowo BUDI SETIABUDIAWAN Budi Setiabudiawan Budiman , Budiman Chrysanti Murad Chrysanti Murad, Chrysanti Cissy B Kartasasmita Cissy B. Kartasasmita Coriejati Rita D Oktavia Dahlan, Zulkarnain Delita Prihatni Dewi Kartika T Dewi Kartika Turbawaty Dewi Lokida Dewi Nurhayati Dick van Soolingen Dida Ahmad Gurnida Djatnika Setiabudi E. Risdiyani Edhyana Sahiratmadja Efrida Efrida Efriyani, Yuhpita Indah Elsa Yulius Endang Samaun Endang Sutedja Erica Catarina Erna Jupri Evan Susandi Fairuz Quzwain Fiona Lestari Fiona Lestari, Fiona Ganiem AR Gde Sindu Mega Hartanto Bayuaji Hendra Subroto, Hendra Herri S. Sastramihardja Herry Garna Hinta Meijerink I. Sjahid Idaningroem Sjahid Ike Sri Redjeki Indahwaty, - Ivana Agnes Sulianto J. E. Gunawan Jamsari Jamsari Johanes C. Mose Johanes C. Mose Julia Hartati Juniati, Rianita Larissa Larissa Lelly Yuniarti Leni Lismayanti Lidya Chaidir Linda Choerunnisa Lisda Amalia Livia Noviani Liza Liza Luhung Budiailmiawan Mike Rezeki Nanan Sekarwana Nataprawira, Heda M Nenny Gustiani Nenny Gustiani Ni Sayu Dewi B. Nida Suraya Nida Suryana Nina Susana Dewi Nina Tristina Noormartany . Nur Atiik Nurizzatun Nafsi Nurlina Sirait Prawoto Prawoto Prayudi Santoso Pujiyanti, Hapsari Rachmat Soelaeman Rahmat Sumantri Ramdan Panigoro Reinout van Crevel Reinout van Crevel RINI SUNDARI RINI SUNDARI, RINI Rita Rachmayanti Rizki Andriyani Rubin Surachno Gondodiputro Rudi Wisaksana Rully Marsis Amirullah Roesli Ryan Bayusantika S. Nilawati Silvia Rachmayati Soeroto, Arto Y Sofiati Dian Sylvia Rachmayati T. M. Sudiro Tiene Rostini Tiene Rostini Tiene Rostini Tiene Rostini, Tiene Tjahjani M. Sudiro Towifah Fauziah Choerunisa Turbawati DK Uni Gamayani Uun Sumardi, Uun Wia Melia Wida Purbaningsih Wulan Ardhana Iswari Wulan Ardhana Iswari, Wulan Ardhana Yani Triyani Yani Triyani Yanti Yanti Yeva Rosana Yovita Hartantri Yusuf Sulaiman Effendi

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